Last year the PRO-IMPLANT Foundation established collaboration with the Federal Center of Traumatology, Orthopedics and Arthroplasty, Barnaul, Russian Federation. Dr. Svetlana Karbysheva visited now Charité for a one month fellowship to perform research projects on diagnosis of PJI.
"I’m a clinical microbiologist from the Federal Center of Arthroplasty of Russian Federation. Recently we have organized workshop in Barnaul, Russia, with Dr. Andrej Trampuz and Dr. Nora Renz as invited speakers, where we discussed and issued the Russian version of Pocket Guide to Diagnosis Treatment of Periprosthetic Joint Infection (PJI). I’m here for the third time. The first time I attended the Workshop on PJI, second time I performed clinical observership and now I am here for three research projects:
1. In the first project we evaluated the role of D-lactate as a pathogen specific marker in synovial fluid in native and prosthetic joints compared to leukocytes. D-lactate has better sensitivity compared to leukocytes, especially in gout and rheumatic diseases. D-lactate has better sensitivity also for diagnosis for PJI. In future studies, D-lactate will be evaluated as a potential diagnostic marker for early postoperative infections and as a diagnostic marker before re-implantations.
2. Another project is to investigate bacterial load to different hip and knee prosthetic components by sonication. Polyethylene showed the highest affinity for biofilm adherence. Therefore, sonication of polyethylene components is sufficient instead of the whole prosthesis.
3. We also aimed to evaluate multiplex PCR for microbiological diagnosis from periprosthetic tissue, sonication and synovial fluid compared to conventional cultures and histopathology. These findings may improve the rate of pathogen identification in patient with implant-associated infections."
Dr. Svetlana Karbysheva, Federal Center of Traumatology, Orthopedics and Arthroplasty, Barnaul, Russian Federation