The aim is to improve understanding of biofilm formation, lead to innovative strategies for detection and eradication of implant infections.
The research is carried out in cooperation with foundations, industry and academic institutions at the Berlin-Brandenburg Center for Regenerative Therapies (BCRT) at Charité – University Medicine Berlin. Research topics are:
Topic 1. New approaches for diagnosis of biofilms by sonication, microcalorimetry (detection of growth-related microbial heat production), molecular techniques (PCR, sequencing), next generation mass spectrometry and atomic force microscopy. Novel techniques are used for early and sensitive detection, rapid identification and susceptibility determination in bacteria and fungi.
Topic 2. Evaluation of investigational antimicrobials against bacterial and fungal biofilms. Pharmacokinetics and activity against planktonic and biofilm microorganisms are evaluated in vitro and in a guinea pig foreign-body infection model. The following organisms are studied: staphylococci, streptococci, enterococci, Escherichia coli, Propionibacterium spp. and Candida spp.
Topic 3. Investigation of release kinetics and biofilm formation on resorbable and non-resorbable biomaterials (PMMA-bone cement, bioactive glass, bone substitutes). Antibiofilm activities of bioactive and impregnated materials are investigated in vitro and in the guinea pig animal model. Innovative approaches such as click chemistry.
Topic 4. Investigation of bacteriophage activity against planktonic and biofilm bacteria, including multiresistant pathogens. Studies are performed in vitro (conventional microbiology, microcalorimetry, imaging) and in vivo (animal model of biofilms). The activity of bacteriophages will be investigated alone and in combination with antibiotics. Target microorganisms are staphylococci, enterococci and gram-negative bacilli.
If you are interested to join the group, cooperate or collaborate, please contact us. We offer a pleasant and stimulating working environment in a dynamic and motivated team, mentorship and integration in clinical projects.
Dr. Andrej Trampuz,
Research Group Leader